Roles of Interleukin-1 (Il-1 ) and Nitric Oxide (No) in the Anti-Inflammatory Dynamics of Acetylsalicylic Acid Against Carrageenan Induced Paw Oedema in Mice

The study aimed at analyzing the role of each of interleukin-1 (IL-1 ) and Nitric Oxide (NO) in development of peripheral acute inflammation in mice in presence/absence of a one hour pre-administered acetylsalicylic acid (ASA). Eighty male mice were utilized. Carrageenan was administered intraplanterly (0.05 ml of 0.5, 1 or 2%, W/V). The examined parameters were paw volume, IL-1 and nitrite levels in plasma and paw infiltrate and the total and differential white blood cells count. Carrageenan administration caused dose-dependent increase tendencies in paw volume, paw infiltrate's IL-1 and plasma nitrite content. Pre-treatment with ASA resulted in augmenting the rise in plasma IL-1 , decreasing IL-1 at the inflammation site and restoring plasma nitrite content to its normal range. The findings indicate that the carrageenan amount is the major determinant of the response to ASA.